Aneeta Pradhan

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Biography

Aneeta has more than 20 years' experience in agricultural research in Australia and overseas. She has made a number of contributions in plant breeding and Molecular Genetics. She has developed DNA fingerprinting keys, the first of its kind in the world, for the identification of radish cultivars. She has successfully created a trigenomic hexaploid, ‘Super Brassica’ from the interspecific hybridisation between B. napus and B. nigra. After completing her PhD at UWA in 2010, Aneeta joined Canola Breeders Western Australia Pty Ltd. for canola crossing programme and in lupin marker project at UWA and CSIRO, where she was involved in developing molecular marker tools for improving the adaptation of wild and domesticated lupin. Aneeta also worked in tedera marker project at UWA and DPIRD, where she was responsible for in-vitro seed germination, the assessment of genetic diversity, the selection of hybrids useful in developing new cultivars, and evaluation of out-crossing rates. In 2016, she joined Batley lab where she is primarily responsible for assessing genetic variation and genotype screening with traits of interest using different techniques of genotyping by sequencing (GBS) methods such as Restriction site Associated DNA Sequencing (RAD-Seq), double digest RAD-seq (ddRAD-Seq), skim sequencing and genome-wide association studies with the Illumina Infinium HD Assay. Currently, her research also involves interspecific crossing among Brassicaceae species to capture useful genes and to produce a population of trigenomic Brassica hexaploids with greater genetic variation to develop a new crop species that may be suitable for vegetables, oilseed crops or condiments which are more vigorous, better adapted and higher yielding than their diploid and tetraploid progenitors.

Research Interests

Plant genetics and genomics, wide hybridisation, phenotypic and genotypic characterisation, trait association, seed testing and quality control

Current Projects

Interspecific and intergeneric hybridisation, polyploid induction, cytogenetics, in-vitro culture, high throughput genotyping

Interspecific hybridisation is a valuable tool for transferring traits from one species to another. Successful interspecific crosses depends on diverse genotype selection and enhanced performance through heterosis. Estimates of genetic diversity and the relationships among germplasm collections are very useful for diverse genotypes selection, identification of variation within traits, making crosses, mapping and potentially for production of new breeding lines/cultivars. The breeding target of Brassica at the hexaploid level has potential for greater disease resistance and wider adaptation to varied environments.

Awards and Scholarships

• Best Advanced Student Presentation Award (2008). The best student presentation at the Rottnest Island Postgraduate Summer School organised by the School of Plant Biology, The University of Western Australia

• Best Student Award (2007). The best student presentation in the 15th Biennial Australian Research Assembly on Brassicas, Geraldton, Western Australia

• UWA University Postgraduate Award: PhD (2006 - 2010)

• AusAid Scholarship: M.Sc. Horticulture (2000 - 2002)

• Colombo Plan Scholarship: B.Sc. (Hons) Agriculture (1988 - 1992)

Publications

Peer-reviewed journal articles

1. Mason AS, Rousseau-Gueutin M, Morice J, Bayer P, Besharat N, Cousin A, Pradhan, A., Parkin IA, Chevre AM, Batley J, Nelson M (2016) Centromere Locations in Brassica A and C Genomes Revealed Through Half-Tetrad Analysis. Genetics 202: 513-523

2. Pradhan A, Besharat N, Castello M, Croser J, Real D, Nelson MN (2014) Evidence for outcrossing in the perennial forage legume tedera (Bituminaria bituminosa). Crop Science 54: 2406-2412 (doi:10.2135/cropsci2014.02.0163)

3. Real D, Oldham CM, Nelson MN, Croser J, Castello M, Verbyla A, Pradhan A, Van Burgel A, Mendez P, Correal E, Teakle NL, Revell CK, Ewing MA (2014) Evaluation and breeding of tedera for Mediterranean climates in southern Australia. Crop and Pasture Science 65:1114-1131 (online: 10.1071/CP13313)

4. Castello M, Croser J, Lulsdorf MM, Ramankutty P, Pradhan A, Nelson MN, Real D (2013) Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C. H. Stirt. vars albomarginata and crassiuscula). Grass and Forage Science (online: 10.1111/gfs.12107)

5. Geng XX, Chen S, Astarini LA, Yan G, Tian E, Meng J, Li ZY, Ge XH, Nelson MN, Mason AS, Pradhan A, Zhou WJ, Cowling WA (2013) Doubled haploids of novel trigenomic Brassica derived from various interspecific crosses. Plant Cell Tissue and Organ culture 113: 501-511

6. Chen S, Nelson MN, Chevre A-M, Jenczewski E, Li Z, Mason AS, Meng J, Plummer JA, Pradhan A, Siddique KHM, Snowdon RJ, Yan G, Zhou W, Cowling WA (2011) Trigenomic bridges for Brassica improvement. Critical Reviews in Plant Sciences 30: 524-547

7. Pradhan A, Nelson MN, Plummer JA, Cowling WA, Yan G (2011) Characterisation of Brassica nigra collections using simple sequence repeat markers reveals distinct groups associated with geographical location, and frequent mislabelling of species identity. Genome 54, 50-63.

8. Pradhan A, Plummer JA, Nelson MN, Cowling WA, Yan G (2010) Successful induction of trigenomic hexaploid Brassica from a triploid hybrid of B. napus L. and B. nigra (L.) Koch. Euphytica 176, 87-98.

9. Pradhan A, Plummer JA, Nelson MN, Cowling WA, Yan G (2010) Trigenomic hybrids from interspecific crosses between Brassica napus and B. nigra. Crop and Pasture Science 61, 464-474.

10. Pradhan A., Yan G, Plummer JA (2004) Correlation of morphological traits with molecular markers in radish (Raphanus sativus). Australian Journal of Experimental Agriculture 44, 813-819.

11. Pradhan A, Yan G, Plummer JA (2004) Development of DNA fingerprinting keys for the identification of radish cultivars. Australian Journal of Experimental Agriculture 44, 95-102.

Refereed conference paper

1. Inturrisi FC, Tirnaz S, Bayer PE, Neik TX, Yang H, Dolatabadian A, Zhang F, Severn-Ellis A, Patel DA, Pradhan A, Lee HT, Edwards D and Batley J (2018) Genome-Wide Analysis of NBS-LRR Genes in the Brassicaceae and Applications for Breeding. PAG XXVI.

2. Mwathi MW, Pradhan A, Batley J, Mason AS (2018). Creating new Brassica oleracea x B. juncea allohexaploids through ovule culture. Plant and Animal Genome Conference. January 11-17th 2018 San Diego, USA.

3. Real D, Oldham C, Nelson MN, Croser J, Castello M, Gherardi S. Finlayson J, Revell CK, Pradhan A, O’Hara G, Correal E (2013) Tedera: from a promising novel species to a commercial pasture option for Mediterranean southern Australia. In: Michalk DL, Millar GD, Badgery WB, Broadfoot KM (eds) 22nd International Grassland Congress: Revitalising grasslands to sustain our communities. CSIRO Publishing and NSW DPI Australia, pp301-303

4. Yan G, Nelson MN, Pradhan A, Mason A, Weerakoon SR, Si P, Plummer J, Cowling W (2009) Progress towards the creation of trigenomic Brassica hexaploid populations. Presented at the 14th Australasian Plant Breeding Conference/11th Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO) Conference, Cairns, Australia. Paper published in SABRAO Journal of Breeding and Genetics Vol. 41, Special supplement 00274.

5. Pradhan A, Yan G, Plummer JA, Cowling WA (2007) Synthesis of hexaploid Brassica from B. napus and B. nigra. M Amjad and WA Cowling (eds), Oral presentation. Paper published in the Conference Proceedings of the 15th Australian Research Assembly on Brassicas (ARAB 15), Department of Agriculture and Food Western Australia, pp. 84-89.

6. Pradhan, A., Plummer J. A., and Yan G. (2004). Comparison of morphological and molecular variation in seeds and seedlings of radish cultivars. Acta Horticulturae 659, 791-798.

Contact Details

Aneeta Pradhan (Research Academic)

School of Biological Sciences

The University of Western Australia

Crawley, WA 6009, Perth, Australia

Phone: +61 8 6488 1557

Email: aneeta.pradhan@uwa.edu.au