Linh Bao Ton
Linh obtained a Bachelor degree in Biotechnology at Nong Lam University, Ho Chi Minh City, Viet Nam and Master's degree in Applied Science at RMIT University, Australia. She conducted research on plant tissue culture and plant transformation, isolation and characterization of Bacillus thuringiensis and identification of plant pathogens such as phytoplasmas. Since 2019, she have joined Batley’s Lab and started her PhD project with Professor Jacqueline Batley.
Gene technology, genomics, plant-pathogen interactions
Turnip mosaic virus (TuMV), a potyvirus, is known to cause significant economic loss in vegetable crops, primarily in the Brassicaceae family. This virus is transmitted into Brassica plants in a non-persistent manner by over 89 aphid species, which limit the effectiveness of chemical control and general treatments. The viral proteins, such as P1 and HC-Pro (helper component protein), are the main factors for the multiplication and spread of TuMV in host plants via suppression of the plant RNA silencing pathway. Within this research, the TuMV in symptomatic canola plants and other Brassica plants will be isolated, pathotyped, and confirmed by PCR, from which P1 and HC-pro genes will be cloned and utilized in the development of genetic modification system for B. napus. As a novel tool of genome editing, the CRISP/Cas system (type II clustered regularly interspaced short palindromic repeats/associated protein system), will be designed to introduce Cas gene, P1 and HC-Pro gene segments into Brassica napus genome. The successfully modified canola lines will be a valuable resource for breeding their resistance against TuMV.
1.Truong, H. P. T., Duong, H. K., Ton, L. B., Le, D. D., Tran, N. T. H., Dang, T. T., and Huynh, L. N. C. (2019). Determination of cry2A genes in Bacillus thuringiensis isolated from southern provinces of Viet Nam. The Journal of Agriculture and Development 18(1), 109-116.
2.Nguyen, P. V., Nguyen, L. T. N., Tran, T. B. and Ton, L. B. (2019). Construction of artificial microRNA expression vectors for inhibition of Minc16281 gene in root-knot nematode Meloidogyne incognita. The Journal of Agriculture and Development 18(4), 62-69.
3.Nguyen, P. V., Ton, L. B, Ngo,T.T.T., Le, V.X. (2018). Determination of some conditions for hairy root induction in soybean by Agrobacterium rhizogenes. Journal of Agricultural and Development 4: 114 – 121.
4.Ton, L. B, Phan, H. T.T, Le, T.T.H, Nguyen, P.V., Ton, A.T., and Le, D.D. (2018). Isolation, identification, and toxicity of native Bacillus thuringiensis against Spodoptera litura. Agrica 7: 66-73
5.Ton, A.T., Ton, L. B., Nguyen, P.V., Bien, T.L.T., To, T.T.N., and Le, D.D. (2018). Expression of proteins related to Phytophthora capsici tolerance in black pepper (Piper nigrum L.). International Journal of Agriculture Innovations and Research 6(4): 75-79
6.Ton, A.T., Ton, L. B., and Le, D.D. (2015). Evaluation of black pepper for resistance to Phytophthora capsici. Proceedings of National conference on Plant protection held at Nong Lam University Ho Chi Minh city on November 6th 2015, pg. 231 – 238
7.Phan,H.T., Ngo, T.T. and Ton, L. B. (2014). Application of temporary immersion system in micropropagation of sugarcane (Saccharum officinarum L.). Journal of Agricultural Science and Technology (2): 29-34
Linh Bao Ton
Room 1.122, School of Biological Sciences, Faculty of Science
The University of Western Australia
Crawley, WA 6009, Perth, Australia